Complete nucleotide sequence and genomic organization of a primate calicivirus, Pan-1

• Published in: Archives of virology Vol. 144; no. 1; pp. 199 - 208
• Main Authors: Rinehart-Kim, J.E, Zhong, W.M, Jiang, X, Smith, A.W, Matson, D.O
• Format: Journal Article
• Language: English
• Published: Wien Springer 1999; New York, NY Springer Nature B.V
• Subjects: Animals; Base Sequence; Biological and medical sciences; Caliciviridae; Caliciviridae - genetics; Cats; DNA, Complementary - genetics; Fundamental and applied biological sciences. Psychology; genbank/af091736; Genetics; Genome, Viral; genomics; Microbiology; Molecular Sequence Data; nucleotide sequences; Pan; Pan paniscus; Primates; Primates - virology; Sequence Analysis; Virology; Virus; Calicivirus; Genome organization; RNA; Caliciviridae; Veterinary; Protein; Structural analysis; Open reading frame
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s007050050497

The primate calicivirus, Pan-1, was originally isolated from several primate species. It displayed typical calicivirus morphology by electron microscopy. We determined the genomic sequence of Pan-1 by cDNA cloning and direct RNA sequencing. Pan-1 shares a similar genomic organization and a high degree of sequence identity with feline caliciviruses. The Pan-1 genome contains 8,304 nucleotides, plus a poly-A tail, and is longer than any other calicivirus strains with a completely known sequence. The extra sequences of Pan-1 include a unique 424-nucleotide sequence at the 5' end of ORF1, additional amino acids at the N-terminus of the capsid, and a longer 3' UTR.