Cloning, Characterization, and Tissue Expression Pattern of Mouse Nma/BAMBI During Odontogenesis

• Published in: Journal of dental research Vol. 80; no. 10; pp. 1895 - 1902
• Main Authors: Knight, C., Simmons, D., Gu, T.T., Gluhak-Heinrich, J., Pavlin, D., Zeichner-David, M., MacDougall, M.
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.10.2001; SAGE PUBLICATIONS, INC
• Subjects: Activins - antagonists & inhibitors; Amino Acids - analysis; Amino Acids - genetics; Animals; Base Pairing - genetics; Blotting, Northern; Bone Morphogenetic Protein 7; Bone Morphogenetic Proteins - genetics; Cell Line; Cloning, Molecular; Conserved Sequence - genetics; Dentistry; DNA, Complementary - genetics; Gene Expression Regulation; Genes, vif - genetics; Genetic Vectors; Humans; In Situ Hybridization; Membrane Proteins - genetics; Mice; Molecular Sequence Data; Odontogenesis - genetics; Plasmids; Rabbits; Reading Frames - genetics; Receptors, Transforming Growth Factor beta - genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Transforming Growth Factor beta - genetics; Xenopus; Xenopus Proteins; epithelial-mesenchymal interactions; Nma; transmembrane protein; odontogenesis; BAMBI
• ISSN: 0022-0345; 1544-0591
• DOI: 10.1177/00220345010800100701

Degenerate oligonucleotides to consensus serine kinase functional domains previously identified a novel, partial rabbit tooth cDNA (Zeichner-David et al., 1992) that was used in this study to identify a full-length mouse clone. A 1390-base-pair cDNA clone was isolated encoding a putative 260-amino-acid open reading frame containing a hydrophobic 25-amino-acid potential transmembrane domain. This clone shares some homology with the TGF-β type I receptor family, but lacks the intracellular kinase domain. DNA database analysis revealed that this clone has 86% identity to a newly isolated human gene termed non-metastatic gene A and 80% identity to a Xenopus cDNA clone termed BMP and activin membrane bound inhibitor. Here we report the mouse Nma/BAMBI cDNA sequence, the tissue expression pattern, and confirmed expression in dental cell lines. This study demonstrates that Nma/BAMBI is a highly conserved protein across species and is expressed at high levels during odontogenesis.