Silk fibroin/methacrylated gelatine/hydroxyapatite biomimetic nanofibrous membranes for guided bone regeneration

• Published in: International journal of biological macromolecules Vol. 263; no. Pt 2; p. 130380
• Main Authors: Li, Bo, Chen, Ying, He, Jisu, Shu, Yue, Yang, Haocheng, Liu, Junhong, Zhang, Chi, Xiao, Wenqian, Liu, Zhongning, Liao, Xiaoling
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2024
• Subjects: animal models; biocompatibility; biomimetics; bone formation; Bone regeneration; fibroins; gelatin; hydroxyapatite; Methacrylated gelatine; nanofibers; Silk fibroin; Bone regeneration; Silk fibroin; Methacrylated gelatine
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.130380

By mimicking in vivo bionic microenvironment and promoting osteogenic differentiation, the hybrid organic-inorganic nanofibrous membranes provide promising potential for guided bone regeneration (GBR) in the treatment of clinical bone defects. To develop a degradable and osteogenic membrane for GBR by combining the natural biomacromolecule silk fibroin (SF) and gelatine with the bioactive nano hydroxyapatite (nHA), the anhydride-modified gelatine-nano hydroxyapatite (GelMA-nHA) composites were synthesized in situ and introduced into silk fibroin to prepare nanofibrous membranes with different ratios using electrospinning and photocrosslinking. The nanofibrous membranes, particularly those with a mass ratio of 7:2:1, were found to exhibit satisfactory elongation at break up to 110 %, maintain the nanofibrous structure for up to 28 days, and rapidly form bone-like apatite within 3 days, thus offering advantages when it comes to guided bone regeneration. In vitro cell results showed that the SF/GelMA/nHA membranes had excellent biocompatibility and enhanced osteogenic differentiation of hBMSCs. In vivo studies revealed that the hybrid composite membranes can improve bone regeneration of critical-sized calvarial defects in rat model. Therefore, the novel hybrid nanofibrous membrane is proposed to be a alternative candidate for creating a bionic microenvironment that promotes bone regeneration, indicating their potential application to bone injury treatment. •SF and GelMA nanofibrous -membranes containing nHA were prepared by electrospinning and photocrosslinking.•This novel nanofibrous membrane has -satisfactory mechanical strength and degradation rate.•This novel biomimetic SF/GelMA/nHA membrane provides a promising strategy to enhance bone regeneration.