Sequence-selective binding of phleomycin to DNA
The binding of phleomycin and bleomycin to DNA has been investigated by studying their effects on cleavage by DNAase I and micrococcal nuclease. In the presence of cobalt, cleavage of DNA by the antibiotics is suppressed, yet they still provide protection from nuclease attack in regions surrounding...
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| Published in | Biochemical journal Vol. 243; no. 3; pp. 847 - 851 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
England
01.05.1987
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The binding of phleomycin and bleomycin to DNA has been investigated by studying their effects on cleavage by DNAase I and micrococcal nuclease. In the presence of cobalt, cleavage of DNA by the antibiotics is suppressed, yet they still provide protection from nuclease attack in regions surrounding the drug cleavage sites. We conclude that cleavage by phleomycin occurs at bonds around which the antibiotic is already selectively bound. |
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| AbstractList | The binding of phleomycin and bleomycin to DNA has been investigated by studying their effects on cleavage by DNAase I and micrococcal nuclease. In the presence of cobalt, cleavage of DNA by the antibiotics is suppressed, yet they still provide protection from nuclease attack in regions surrounding the drug cleavage sites. We conclude that cleavage by phleomycin occurs at bonds around which the antibiotic is already selectively bound. The binding of phleomycin and bleomycin to DNA has been investigated by studying their effects on cleavage by DNAase I and micrococcal nuclease. In the presence of cobalt, cleavage of DNA by the antibiotics is suppressed, yet they still provide protection from nuclease attack in regions surrounding the drug cleavage sites. The authors conclude that cleavage by phleomycin occurs at bonds around which the antibiotic is already selectively bound. |
| Author | Waring, M J Fox, K R Grigg, G W |
| AuthorAffiliation | Department of Pharmacology, University of Cambridge, U.K |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/2444208$$D View this record in MEDLINE/PubMed |
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| Snippet | The binding of phleomycin and bleomycin to DNA has been investigated by studying their effects on cleavage by DNAase I and micrococcal nuclease. In the... |
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| SubjectTerms | Autoradiography Base Sequence Binding Sites Bleomycin - metabolism Cobalt Deoxyribonuclease I DNA - metabolism Micrococcal Nuclease Phleomycins - metabolism |
| Title | Sequence-selective binding of phleomycin to DNA |
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