Sequence-selective binding of phleomycin to DNA

The binding of phleomycin and bleomycin to DNA has been investigated by studying their effects on cleavage by DNAase I and micrococcal nuclease. In the presence of cobalt, cleavage of DNA by the antibiotics is suppressed, yet they still provide protection from nuclease attack in regions surrounding...

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Bibliographic Details
Published inBiochemical journal Vol. 243; no. 3; pp. 847 - 851
Main Authors Fox, K R, Grigg, G W, Waring, M J
Format Journal Article
LanguageEnglish
Published England 01.05.1987
Subjects
Autoradiography
Base Sequence
Binding Sites
Bleomycin - metabolism
Cobalt
Deoxyribonuclease I
DNA - metabolism
Micrococcal Nuclease
Phleomycins - metabolism
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Summary:The binding of phleomycin and bleomycin to DNA has been investigated by studying their effects on cleavage by DNAase I and micrococcal nuclease. In the presence of cobalt, cleavage of DNA by the antibiotics is suppressed, yet they still provide protection from nuclease attack in regions surrounding the drug cleavage sites. We conclude that cleavage by phleomycin occurs at bonds around which the antibiotic is already selectively bound.
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ISSN:0264-6021
1470-8728
DOI:10.1042/bj2430847