Rubritalea profundi sp. nov., isolated from deep-seawater and emended description of the genus Rubritalea in the phylum Verrucomicrobia

• Published in: International journal of systematic and evolutionary microbiology Vol. 68; no. 4; pp. 1384 - 1389
• Main Authors: Song, Jaeho, Lim, Yeonjung, Joung, Yochan, Cho, Jang-Cheon, Kogure, Kazuhiro
• Format: Journal Article
• Language: English
• Published: England 01.04.2018
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.002686

A Gram-stain-negative, short-rod, facultatively anaerobic, non-motile and red-pigmented bacterium, designated SAORIC-165 , was isolated from a deep-seawater sample collected from the Pacific Ocean. The 16S rRNA gene sequence analysis showed that strain SAORIC-165 was most closely related to Rubritalea marina Pol012 (95.7 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Rubritalea in the phylum Verrucomicrobia. Optimal growth of strain SAORIC-165 was observed at 10 °C, pH 7.0 and in the presence of 2.0-3.5 % (w/v) NaCl. The DNA G+C content of strain SAORIC-165 was 50.7 mol% and MK-9 was the predominant isoprenoid quinone. The major cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C14 : 0, anteiso-C15 : 0, C16 : 0 and C14 : 0. The major polar lipids constituted phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified phospholipids and aminolipids. On the basis of the taxonomic data obtained in this study, it was concluded that strain SAORIC-165 represented a novel species of the genus Rubritalea, for which the name Rubritalea profundi sp. nov. is proposed. The type strain of Rubritalea profundi is SAORIC-165 (=NBRC 110691 =KCTC 52460 ).