Entamoeba histolytica: intracellular distribution of the sec61α subunit of the secretory pathway and down-regulation by antisense peptide nucleic acids
The Sec61α protein is defined as a highly conserved essential integral component of the endoplasmic reticulum in eukaryotic cells. We report a detailed immunolocalization of the Entamoeba histolytica homologue of the Sec61α subunit ( EhSec61α), which shows an irregular pattern throughout the cell an...
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Published in | Experimental parasitology Vol. 109; no. 4; pp. 241 - 251 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
San Diego, CA
Elsevier Inc
01.04.2005
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | The Sec61α protein is defined as a highly conserved essential integral component of the endoplasmic reticulum in eukaryotic cells. We report a detailed immunolocalization of the
Entamoeba histolytica homologue of the Sec61α subunit (
EhSec61α), which shows an irregular pattern throughout the cell and is also found on the cell surface, its effective down-regulation by means of antisense peptide nucleic acids and its effects on cell proliferation, subcellular distribution of two virulence factors, and the ability of the trophozoites to cause liver abscess in hamsters. Although Sec61α levels are specifically decreased in antisense PNA-treated trophozoites, which proliferate more slowly than the controls, mobilization of the cysteine protease 5 and amoebapore to the cell surface is not significantly impeded and the capacity to induce liver abscess in hamsters is largely unaffected. The implications of these findings are discussed in the context of the peculiar cell biology of
E. histolytica. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0014-4894 1090-2449 |
DOI: | 10.1016/j.exppara.2004.12.011 |