Facile construction of electrospun zein nanofiber loaded with Rana chensinensis skin collagen for wound care after caesarean section surgery

Abstract Numerous types of biomedical applications have found success using electrospun nanofibers. However, electrospinning techniques overlook Zein fibers favoring monolithic and coaxial fibers. Uniaxial electrospinning was used to construct Zein-structured nanofibers for wound healing, with the p...

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Bibliographic Details
Published inMaterials research express Vol. 10; no. 1; pp. 15404 - 15416
Main Authors Zhu, Lingping, Lin, Chenxiao, Chen, Qiaoling
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.01.2023
Subjects
Biomedical materials
Collagen
E coli
Electrospinning
electrospun
Mechanical properties
Microorganisms
Nanofibers
Nanoparticles
Phase separation
Polycaprolactone
Polyvinyl alcohol
rana chensinensis skin collagen
Silver
silver nanoparticles
Surgery
Thermal conductivity
Wettability
Wound healing
wound scratch
Zein
zein nanofibers
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Summary:Abstract Numerous types of biomedical applications have found success using electrospun nanofibers. However, electrospinning techniques overlook Zein fibers favoring monolithic and coaxial fibers. Uniaxial electrospinning was used to construct Zein-structured nanofibers for wound healing, with the phase separation of electrospun polycaprolactone (PCL)/polyvinyl alcohol (PVA) in solution, providing the basis for the investigation. Successful loading of silver nanoparticles (AgNPs) and Rana chensinensis skin collagen (RCSCs) into Zein nanofibers (NFs) improved their bioactivity and antibacterial activity as wound dressings. Zein nanofibers were investigated for their heat conductivity, wettability, and mechanical characteristics. The cytotoxic effects of fabricated nanofibers were examined by using L929 fibroblast cells. Ag@RCSCs-NFs also increased cell migration and proliferation, and the wound scratch model was significantly reduced in size using an in vitro scratch assay. The antibacterial studies showed that the Ag@RCSCs-NFs have a potent antibacterial effect against the tested microbial pathogens ( Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus )). Finally, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PCL/PVP, RCSCs, and Ag@RCSCs against two different bacteria were determined. These results suggest that wound care using Zein nanofibers loaded with RCSCs and AgNPs during cesarean section surgery has great promise.
Bibliography:MRX-126148.R1
ISSN:2053-1591
2053-1591
DOI:10.1088/2053-1591/ac99bc