Wickerhamiella osmotolerans sp. nov. and Wickerhamiella tropicalis sp. nov., novel ascomycetous yeast in the family Wickerhamiellaceae

• Published in: International journal of systematic and evolutionary microbiology Vol. 70; no. 4; pp. 2596 - 2601
• Main Authors: Sakpuntoon, Varunya, Angchaun, Jirameth, Boonmak, Chanita, Chang, Chin-Feng, Liu, Shiu-Mei, Lee, Ching-Fu, Limtong, Savitree, Srisuk, Nantana
• Format: Journal Article
• Language: English
• Published: England 01.04.2020
• Subjects: novel yeast species; ascomycetous yeast; grease trap; osmotolerant yeast
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.004075

Seven yeast strains, DMKU VGT1-14 , DMKU VGT1-19 , DMKU-JMGT1-28, DMKU-JMGT1-32, DMKU VGT2-06, DMKU VGT2-19 and DMKU VGT6-14, were isolated from a grease trap in Thailand and two strains, SJ-1 and SN-102 were isolated from the sea surface microlayer in Taiwan. On the basis of phenotypic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, these strains represented two novel yeast species of the genus . In terms of pairwise sequence similarity, four strains, DMKU VGT1-14, DMKU-JMGT1-32, DMKU VGT6-14 and SN-102, were closely related to NRRL Y-17858 but differed by 13 nucleotide substitutions with one gap (2.46 %) in the D1/D2 domain of the LSU rRNA gene and 15 nucleotide substitutions with 23 gaps (4.2 %) in the ITS region. The strains DMKU VGT1-19 , DMKU-JMGT1-28, DMKU VGT2-06, DMKU VGT2-19 and SJ-1, differed from the type strain of the most closely related species, NRRL Y-7921 , by nine nucleotide substitutions with one gap (1.66 %) in the D1/D2 domain of the LSU rRNA gene and nine nucleotide substitutions with 17 gaps (2.52%) in the ITS region. Hence, the names sp. nov. and sp. nov. are proposed to accommodate these species in the genus . The holotypes are DMKU VGT1-14 (ex-type strain TBRC 11425=PYCC 8359=CGMCC 2.6179; Mycobank number 833394) and DMKU VGT1-19 (ex-type strain TBRC 11426=PYCC 8360=CGMCC 2.6180; Mycobank number 833393).