Graphical and statistical analysis of hyperbolic tight-binding inhibition

Equations are described for the analysis of enzyme-catalysed reactions in the presence of hyperbolic tight-binding inhibitors using both graphical and non-linear-regression methods. The general character of the equations allows the interpretation of several other kinetic mechanisms. As an example, t...

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Bibliographic Details
Published inBiochemical journal Vol. 244; no. 3; pp. 793 - 796
Main Author Baici, A
Format Journal Article
LanguageEnglish
Published England 15.06.1987
Subjects
elastase
Enzyme Inhibitors
glycosaminoglycans
Glycosaminoglycans - pharmacology
Humans
Kinetics
leukocytes
Leukocytes - enzymology
Models, Biological
Pancreatic Elastase - antagonists & inhibitors
Pancreatic Elastase - blood
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Summary:Equations are described for the analysis of enzyme-catalysed reactions in the presence of hyperbolic tight-binding inhibitors using both graphical and non-linear-regression methods. The general character of the equations allows the interpretation of several other kinetic mechanisms. As an example, the tight-binding hyperbolic non-competitive inhibition of human leucocyte elastase by a polysulphated glycosaminoglycan is analysed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2440793