Cloning, expression and functional characterization of recombinant tumor necrosis factor α1 (TNFα1) from white crucian carp in gut immune regulation

• Published in: International journal of biological macromolecules Vol. 254; no. Pt 1; p. 127770
• Main Authors: Li, Shi-Yun, Xiong, Ning-Xia, Li, Ke-Xin, Huang, Jin-Fang, Ou, Jie, Wang, Fei, Huang, Ming-Zhu, Luo, Sheng-Wei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2024
• Subjects: Aeromonas hydrophila; Antioxidant; antioxidants; atrophy; Carassius; caspases; Crucian carp; edema; farmed fish; homeostasis; Immune regulation; immunomodulation; lipopolysaccharides; midgut; mucins; mucosal immunity; pathogenesis; pleiotropy; superfamily; tight junctions; TNFα; tumor necrosis factors; Crucian carp; TNFα; Immune regulation; Antioxidant
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2023.127770

TNFα is one of important cytokines belonging to TNF superfamily, which can exhibit a pleiotropic effect in immune modulation, homeostasis as well as pathogenesis. However, its immunoregulatory function on mucosal immunity in fish gut are still unclear. In this study, we aimed to investigated the immunoregulatory role of TNFα1 in midgut of white crucian carp (WCC). WCC-TNFα1 sequence and its deduced structure were firstly identified in WCC. Then, tissue-specific analysis revealed that high-level WCC-TNFα1 expression was detected in gill. After Aeromonas hydrophila and lipopolysaccharide (LPS) stimulated, increased trends of WCC-TNFα1 expressions were detected in immune-related tissues and cultured fish cells, respectively. WCC anal-intubated with WCC-TNFα1 fusion protein showed the increased levels of edema and fuzzy appearance in impaired villi, along with atrophy and reduction of goblet cells (GC). Moreover, the expression levels of tight junction (TJ) genes and mucin genes were consistently lower than those of the control (P < 0.05). WCC-TNFα1 treatment could sharply decrease antioxidant status in midgut, while the expression levels of caspase (CASP) genes, unfolded protein response (UPR) genes and redox response genes increased dramatically. Our results suggested that WCC-TNFα1 could exhibit a detrimental effect on antioxidant and mucosal immune regulation in midgut of WCC. •The deduced structure and expression patterns of WCC-TNFα1 were firstly reported in WCC.•WCC receiving WCC-TNFα1 perfusion showed a dramatically reduced expressions of tight junction and mucin.•WCC-TNFα1 perfusion induced apoptotic activation but decreased antioxidant status in midgut