Simple and Rapid Screening Method for Photosystem II Inhibitory Herbicides Using Photoautotrophically Cultured Plant Cells with Chlorophyll Fluorescence Monitoring

• Published in: Bioscience, biotechnology, and biochemistry Vol. 57; no. 8; pp. 1389 - 1390
• Main Authors: Yoneyama, Koichi, Nakajima, Yoshihiro, Maejima, Noboru, Ogasawara, Masaru, Konnai, Makoto, Tokutake, Nobuya, Iwamura, Hajime, Sato, Fumihiko, Ichinose, Katsunori, Asami, Tadao, Yoshida, Shigeo
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 1993; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; Chemical control; Fundamental and applied biological sciences. Psychology; Parasitic plants. Weeds; Phytopathology. Animal pests. Plant and forest protection; Weeds; Cell culture; Plant juvenile growth stage; Fluorescence; Autotrophy; Spinacia oleracea; Thylakoid; Hepaticae; Electron transfer inhibitor; Photosystem 2; Dicotyledones; Angiospermae; Experimentation; Ureas; Greenhouse study; Monitoring; Chlorophyll; Bioassay; Vigna radiata; Bryophyta; In vitro; Biological activity; Herbicide; Chenopodiaceae; Grain legume; Screening; Leguminosae; Vegetable crop; Test plant; Photoautotrophy; Spermatophyta; Photosynthesis
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.57.1389

The photosystem II inhibitory activity of substituted phenylurea derivatives in liverwort photoautotrophically cultured cells was determined by monitoring chlorophyll fluorescence. Since the inhibitory activities in this assay correlated well with both the Hill inhibitory activity in isolated thylakoids and the herbicidal activity against mung bean plants, this bioassay may be a suitable screening method for photosystem II inhibitory herbicides.