Comparison of Biochemical Effects Induced by Changle between Male and Female Rats Using NMR and ICP-MS Techniques

• Published in: Journal of rare earths Vol. 24; no. 1; pp. 108 - 114
• Main Author: 吴惠丰 李晓晶 冯江华 李伟生 李中峰 廖沛球 吴亦洁 裴奉奎
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.02.2006; Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
• Subjects: biofluid; Changle; ICP-MS; metabolism; NMR; rare earths; 新陈代谢; 生物化学; biofluid; NMR; metabolism; ICP-MS; Changle; rare earths
• ISSN: 1002-0721; 2509-4963
• DOI: 10.1016/S1002-0721(06)60075-6

Metabolic profiles caused by rare earth complex were investigated using NMR and ICP-MS techniques. Male and female Wistar rats were treated orally with Changle （A kind of rare earth complex applied in agriculture to raise the production of crops） at dose of 2, 5 and 20 mg·kg^-1 body weight/day respectively for 90 d. Urine and serum samples are collected on 90 d. The relative concentrations of important endogenous metabolites in urine and serum are determined from ^1H NMR spectra and the contents of the four rare earth elements （La, Ce, Pr and Nd） constituting Changle in the serum samples are measured by ICP-MS technique. Changle-induced renal and liver damage in rats is found based on the increase in the amounts of the amino acids, trimethylamine N-oxide, N, N-dimethyglycine, dimethylamine, succinate, α- ketoglutarate and ethanol as well as rare earth concentrations. The similarities and differentiations are found in the alteration patterns of metabolites and rare earth concentrations in serum.