Simultaneous determination of twelve dyes in meat products: Development and validation of an analytical method based on HPLC-UV-diode array detection

• Published in: Food chemistry Vol. 285; pp. 1 - 9
• Main Authors: Iammarino, Marco, Mentana, Annalisa, Centonze, Diego, Palermo, Carmen, Mangiacotti, Michele, Chiaravalle, Antonio Eugenio
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2019
• Subjects: acetonitrile; ammonia; azo dyes; erythrosine; European Union; food colorants; Food colorings; Food dyes; Food safety; Fresh meat preparations; high performance liquid chromatography; HPLC; meat; Meat products; methanol; solvents; Sudan dyes; ultrasonics; UV-diode array detection; Validation; Validation; Ponceau SX (PubChem CID: 9595288); Amaranth (PubChem CID: 5359521); Food dyes; Sudan I (PubChem CID: 5858445); Food colorings; Carmine (PubChem CID: 14950); Sudan II (PubChem CID: 5809936); Erythrosine (PubChem CID: 12961638); Fresh meat preparations; Ponceau 4R (PubChem CID: 9570119); Allura Red AC (PubChem CID: 5360805); Carmoisine (PubChem CID: 6321394); UV-diode array detection; Ponceau 3R (PubChem CID: 5359885); Food safety; Meat products; HPLC
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2019.01.133

•A novel/versatile HPLC method was studied for the determination of 12 dyes in meat.•The method was fully validated.•Method accuracy resulted in compliance with the actual reference values.•Method sensitivity, selectivity and ruggedness were satisfactory.•The expanded measurement uncertainties were in the range 6.4%–19.5%. The use of food dyes in meat is subject to regulations, due to food safety concerns. A reliable method for the determination of 12 food dyes (Amaranth, Ponceau 4R, Carmine, Ponceau SX, Ponceau 3R, Allura Red AC, Carmoisine, Erythrosine, Sudan I, Sudan II, Sudan III and Sudan IV) in meat products using high performance liquid chromatography coupled to UV-diode array detection was developed, optimized and fully validated. The extraction was accomplished using acetonitrile, methanol, water, ammonia, 50:40:9:1 (v/v/v/v) as the solvent, and an ultrasonic bath. Chromatographic separation was achieved using a C18 RP column and samples eluted with a gradient acetate-acetonitrile mobile phase. Good analytical performance was obtained, in terms of selectivity, sensitivity, accuracy and ruggedness. Both method precision (CV% range: 6.2%–18.0%) and recovery (range: 86.4%–105.0%) complied with Decision 657/2002/EC, suggesting the procedure could be applied successfully for analyses of meat products in the European Union.