Development of a competitive double antibody lateral flow assay for the detection of antibodies specific to glycoprotein B of Aujeszky's disease virus in swine sera

• Published in: Journal of virological methods Vol. 240; pp. 54 - 62
• Main Authors: Vrublevskaya, V.V., Afanasyev, V.N., Grinevich, A.A., Skarga, Yu. Y., Gladyshev, P.P., Ibragimova, S.A., Krylsky, D.V., Morenkov, O.S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2017
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Viral - blood; Antibody detection; Antigens, Viral - immunology; Aujeszky's disease virus; Competitive lateral flow assay; Enzyme-Linked Immunosorbent Assay; Glycoprotein B; Glycoproteins - immunology; Herpesviridae; Herpesvirus 1, Suid - immunology; Herpesvirus 1, Suid - isolation & purification; Neutralization Tests; Pseudorabies - diagnosis; Pseudorabies - immunology; Sensitivity and Specificity; Swine; Swine Diseases - diagnosis; Swine Diseases - immunology; Viral Envelope Proteins - immunology; Antibody detection; Aujeszky's disease virus; Glycoprotein B; Competitive lateral flow assay
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2016.11.011

•A lateral flow assay for the detection of antibodies to Aujeszky's disease virus glycoprotein B.•Competitive sandwich gB-LFA provides specific and sensitive detection of ADV-infected swine. Three lateral flow assays (LFAs) for the detection of antibodies against glycoprotein B (gB) of Aujeszky's disease virus (ADV) in swine sera: a competitive double antibody sandwich LFA without a preincubation step (CDAS-gB-LFA), a CDAS-gB-LFA with a preincubation step (pCDAS-gB-LFA), and a competitive direct gB-LFA have been developed and were compared with each other and with a gB-ELISA. The assays are based on monoclonal antibodies to immunodominant epitopes of ADV gB. The pCDAS-gB-LFA proved to be the most specific and sensitive assay to detect antibodies directed to ADV gB. The specificity and sensitivity of the pCDAS-gB-LFA with the use of an LFA reader for test line intensity measurements were 97.6 and 94.9%, respectively. The lower diagnostic sensitivity of the pCDAS-gB-LFA compared to a gB-ELISA reflects its reduced analytical sensitivity, which was shown in titration experiments with positive sera. The pCDAS-gB-LFA, using the reader-based and visual detection modes, showed good agreement in respect to specificity; however, the LFA reader detection provided a higher diagnostic and analytical sensitivity compared to visual detection. The developed pCDAS-gB-LFA is a rapid, sensitive, and specific method for the detection of antibodies to ADV gB and can be used for screening ADV-infected swine in unvaccinated herds.