An immunochromatographic assay utilizing magnetic nanoparticles to detect major peanut allergen Ara h 1 in processed foods

• Published in: Food chemistry Vol. 375; p. 131844
• Main Authors: Yin, Hsin-Yi, Li, Yi-Ting, Tsai, Wen-Che, Dai, Hong-Yu, Wen, Hsiao-Wei
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2022
• Subjects: 2S Albumins, Plant; Allergens; Antigens, Plant; Ara h 1; Arachis; chocolate; cookies; detection limit; Enzyme-Linked Immunosorbent Assay; food chemistry; Glycoproteins; Humans; immunoaffinity chromatography; Immunoassay; Immunomagnetic nanoparticle; Lateral flow assay; magnetism; Magnetite Nanoparticles; milk; Peanut allergen; Peanut Hypersensitivity; peanuts; Plant Proteins; polysorbates; Lateral flow assay; Peanut allergen; Immunomagnetic nanoparticle; Ara h 1

[Display omitted] •An IMNP-based LFA was developed for detecting Ara h1 in processed foods.•The assay had good specificity in identifying peanut from various seeds and nuts.•The visual detection limit of this assay is 1 μg/g of Ara h1 in chocolate.•The results of this assay closely agreed (97.6%) wi...