An immunochromatographic assay utilizing magnetic nanoparticles to detect major peanut allergen Ara h 1 in processed foods

• Published in: Food chemistry Vol. 375; p. 131844
• Main Authors: Yin, Hsin-Yi, Li, Yi-Ting, Tsai, Wen-Che, Dai, Hong-Yu, Wen, Hsiao-Wei
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2022
• Subjects: 2S Albumins, Plant; Allergens; Antigens, Plant; Ara h 1; Arachis; chocolate; cookies; detection limit; Enzyme-Linked Immunosorbent Assay; food chemistry; Glycoproteins; Humans; immunoaffinity chromatography; Immunoassay; Immunomagnetic nanoparticle; Lateral flow assay; magnetism; Magnetite Nanoparticles; milk; Peanut allergen; Peanut Hypersensitivity; peanuts; Plant Proteins; polysorbates; Lateral flow assay; Peanut allergen; Immunomagnetic nanoparticle; Ara h 1
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2021.131844

[Display omitted] •An IMNP-based LFA was developed for detecting Ara h1 in processed foods.•The assay had good specificity in identifying peanut from various seeds and nuts.•The visual detection limit of this assay is 1 μg/g of Ara h1 in chocolate.•The results of this assay closely agreed (97.6%) with an AOAC-approved ELISA kit. This study describes an immunomagnetic nanoparticle (IMNP)-based lateral flow assay (LFA) for detecting the major peanut allergen Ara h 1. We developed a clearly specific method in identifying peanut from ten other seeds and nuts, and a good visual limit of detection (vLOD) of 0.01 μg/mL Ara h 1 in PBS. PBS that contains 1 M NaCl and 2% Tween 20 was determined to be the optimal extraction buffer for isolating Ara h 1 from cookie, milk and chocolate with vLOD values of 0.5 μg/g, 0.5 μg/mL, and 1 μg/g, respectively. Forty two processed foods were simultaneously analyzed using this method and an AOAC-approved ELISA kit. The specificity and sensitivity of this assay were thus determined to be 100 and 95%, respectively. This new IMNP-based LFA has potential as a rapid tool for screening processed foods for Ara h 1 residues.