Effect of Crab Shell Size on Bio-demineralization with Lactic Acid-Producing Bacterium, Lactobacillus paracasei subsp. tolerans KCTC-3074

• Published in: Biotechnology and bioprocess engineering Vol. 13; no. 5; pp. 566 - 570
• Main Authors: Oh, K.T. (Chonnam National University, Gwangju, Republic of Korea), Kim, Y.J. (Chonnam National University, Gwangju, Republic of Korea), Nguyen, Van Nam (Chonnam National University, Gwangju, Republic of Korea), Jung, W.J. (Chonnam National University, Gwangju, Republic of Korea), Park, R.D. (Chonnam National University, Gwangju, Republic of Korea), E-mail: rdpark@chonnam.ac.kr
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society for Biotechnology and Bioengineering 01.10.2008; Springer Nature B.V; 한국생물공학회
• Subjects: Acidity; Acids; Bacteria; bio-demineralization; Biotechnology; By products; Chemistry; Chemistry and Materials Science; crab shell waste; Crustaceans; Decapoda; DEMINERALISATION; DEMINERALIZATION; DESMINERALIZACION; Fermentation; Glucose; Industrial and Production Engineering; Lactobacillus paracasei; Lactobacillus paracasei subsp. tolerans KCTC-3074; Particle size; Proteins; Shellfish; Statistical analysis; Studies; total titratable acidity; Variance analysis; 생물공학; Lactobacillus paracasei; tolerans
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-008-0007-6

Demineralization (DM) from crab shell (CS) waste was carried out using a lactic acid-producing bacterium, Lactobacillus paracasei subsp. tolerans KCTC-3074 for 7 days at 25, 30, and 35℃. DM rates were 89~92% and slightly affected by temperature. DM was also performed for four particle-sized shell samples (0.84~3.35, 3.35~10, 10~20, and 20~35 mm) with 10% inoculum, 5% shell, and 10% glucose at 30℃ and 180 rpm for 7 days. It was found out that the shell size had a slight effect on the rate of DM. Negative relationships were found between DM and residual dry weight (r² = 0.960), and between DM and pH (r² = 0.906). Conversely, positive relationships were found between DM and medium protein (r² = 0.696), and between DM and total titratable acidity (r² = 0.630).