Molecular recognition based rapid diagnosis of immunoglobulins via proteomic profiling of protein-nanoparticle complexes

• Published in: International journal of biological macromolecules Vol. 138; pp. 156 - 167
• Main Authors: Dar, Aqib Iqbal, Walia, Shanka, Acharya, Amitabha
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2019
• Subjects: Adsorption; biomedical research; Blood Chemical Analysis - methods; chitosan; Chitosan - chemistry; ELISA; Humans; immunoglobulins; Immunoglobulins - blood; Immunoglobulins - chemistry; Immunoglobulins - metabolism; LC-MS; Magneto-fluorescent nanoparticles; Magnets - chemistry; Materials Testing; Nanoparticles - chemistry; polymers; Protein corona; Protein Structure, Secondary; Proteomics; Spectroscopy; Surface Properties; Time Factors; Spectroscopy; LC-MS; Protein corona; Magneto-fluorescent nanoparticles; ELISA
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2019.07.079

Protein-nanoparticle (NP) interaction, which inevitably form protein corona (PC), has been the subject of much debate about its role in modern biomedical research. In this regard, PC associated with two different NPs viz., magneto-fluorescent (MF) and chitosan coated MF (CMF) NPs were thoroughly investigated, to analyze the effect of polymer coating on protein adsorption. Bradford assay, along with the spectroscopic and microscopic studies suggested increase in adsorbed protein quantity, though the results varied significantly on moving from bare to polymeric coating and in vitro to ex vivo conditions. Interestingly, polymer coated NPs showed increased protein adsorption and induce minimal changes in protein structural integrity under the same conditions. We predict that the changes in secondary structure of primary corona determine the overall signature of surface binding proteins in PC. Our findings suggested that rapid diagnosis of immunoglobulins is possible using the concept of protein corona formation ex vivo. A detailed investigation into the protein corona of bare and polymeric coated NPs has been performed to ascertain their comparative immunogenic responses under in vitro and ex vivo conditions using different spectroscopic and microscopic techniques. [Display omitted]