Regulation of Peripheral Spindle Movement and Spindle Rotation during Mouse Oocyte Meiosis: New Perspectives

Spindle movement, including spindle migration during first meiosis and spindle rotation during second meiosis, is essential for asymmetric divisions in mouse oocytes. Previous studies by others and us have shown that microfilaments are required for both spindle migration and rotation. In the present...

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Published inMicroscopy and microanalysis Vol. 14; no. 4; pp. 349 - 356
Main Authors Ai, Jun-Shu, Wang, Qiang, Yin, Shen, Shi, Li-Hong, Xiong, Bo, OuYang, Ying-Chun, Hou, Yi, Chen, Da-Yuan, Schatten, Heide, Sun, Qing-Yuan
Format Journal Article
LanguageEnglish
Published New York, USA Cambridge University Press 01.08.2008
Oxford University Press
Subjects
Animals
Calcium - metabolism
Chemicals
Eggs
Ethanol
Experiments
Meiosis
Mice
Microscopy
Microscopy, Fluorescence
Microtubules - metabolism
Movement
Oocytes - physiology
Rotation
Spindle Apparatus
St Louis Missouri
United States > US
microtubule
spindle rotation
spindle migration
calcium
mouse oocyte
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Summary:Spindle movement, including spindle migration during first meiosis and spindle rotation during second meiosis, is essential for asymmetric divisions in mouse oocytes. Previous studies by others and us have shown that microfilaments are required for both spindle migration and rotation. In the present study, we aimed to further investigate the mechanism controlling spindle movement during mouse oocyte meiosis. By employing drug treatment and immunofluorescence microscopy, we showed that dynamic microtubule assembly was involved in both spindle migration and rotation. Furthermore, we found that the calcium/CaM/CaMKII pathway was important for regulating spindle rotation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1431-9276
1435-8115
DOI:10.1017/S1431927608080343