Characterization of Monocyte-Derived Dendritic Cells in Recent-Onset Diabetes Mellitus Type 1

• Published in: Clinical immunology (Orlando, Fla.) Vol. 105; no. 1; pp. 17 - 24
• Main Authors: Zacher, Thorsten, Knerr, Ina, Rascher, Wolfgang, Kalden, Joachim R., Wassmuth, Ralf
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.10.2002; Elsevier
• Subjects: Adolescent; Antigens, CD - immunology; autoimmunity; Biological and medical sciences; Child; Child, Preschool; Cohort Studies; dendritic cell; Dendritic Cells - cytology; Dendritic Cells - immunology; diabetes mellitus type 1; Diabetes Mellitus, Type 1 - immunology; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; Flow Cytometry; Humans; Interleukin-1 - biosynthesis; Interleukin-1 - immunology; Interleukin-6 - biosynthesis; Interleukin-6 - immunology; Male; Medical sciences; monocyte; Monocytes - cytology; Monocytes - immunology; Statistics, Nonparametric; T-Lymphocytes - immunology; monocyte; autoimmunity; dendritic cell; diabetes mellitus type 1; Endocrinopathy; Human; Autoimmunity; Immunopathology; Dendritic cell; Flow cytometry; Monocyte; Pathogenesis; Non insulin dependent diabetes; Child
• ISSN: 1521-6616; 1521-7035
• DOI: 10.1006/clim.2002.5265

Dendritic cells (DC) may play an important role in the immunopathogenesis of type 1 diabetes mellitus (DM-1). In this study, we have analyzed phenotypical changes during cytokine-driven maturation from CD14 + monocytes to mature DC and DC-dependent T-cell stimulation in recent-onset pediatric DM-1 patients and healthy controls. DC maturation was monitored by flow cytometric analyses for the expression of surface markers (HLA-DR, CD1a, CD40, CD80, CD86, CD83, CD14, CD32, mannose-receptor, and CD11c). Flow cytometric analysis of isolated peripheral blood monocytes did not reveal apparent differences between patients and controls. During DC maturation no obvious differences in the expression patterns of surface markers over time or evidence for maturation impairments in DM-1 patients could be appreciated. Solely, a marginal, but significant, transient downregulation of CD1a on Day 3 (mean MDFI 3.82 vs 7.25; P = 0.021), which was accompanied by an increase of IL-6, could be observed. The comparison of mature DCs (Day 10) between patients and controls indicated no significant differences, except for CD83 (mean MDFI 1.7 vs 1.5; P = 0.042) and CD80 (mean MDFI 15.92 vs 12.73; P = 0.042). Moreover, no difference in T-cell stimulatory capacity was seen. In conclusion, our analysis of a cohort of recent-onset DM-1 patients and controls does not support a role for disease-related alterations in cytokine-driven maturation of monocyte-derived DC.