Isolation and characterization of an AHL lactonase gene from Bacillus amyloliquefaciens

An AHL lactonase gene (aiiA) was PCR amplified from the genomic DNA of Bacillus amyloliquefaciens, with the intact open reading frame of 753 base pair. The gene shares high identity to its homologues present in different Bacillus species. The expression plasmid carrying a tact aiiA-PEBA gene was con...

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Published inWorld journal of microbiology & biotechnology Vol. 26; no. 8; pp. 1361 - 1367
Main Authors Yin, Xiang-Tian, Xu, Liang, Fan, Su-Su, Xu, Li-Na, Li, Duo-Chuan, Liu, Zhen-Yu
Format Journal Article
LanguageEnglish
Published Dordrecht Dordrecht : Springer Netherlands 01.08.2010
Springer Netherlands
Springer
Springer Nature B.V
Subjects
aiiA gene
Applied Microbiology
Bacillus
Bacillus amyloliquefaciens
Bacteria
Bacterial diseases
Biochemistry
Biological and medical sciences
Biological control
biological control agents
Biomedical and Life Sciences
Biotechnology
Cellular biology
Cloning
Daucus
Disease control
E coli
Environmental Engineering/Biotechnology
Enzymes
Escherichia coli
Fundamental and applied biological sciences. Psychology
Gene expression
Laboratories
Life Sciences
Microbiology
Original Paper
Pectobacterium
Plant resistance
Plasmids
Quorum quenching
Studies
Virulence
gene
Quorum quenching
Biocontrol agent
aiiA gene
Enzyme
Biological control
Esterases
Bacillus amyloliquefaciens
Bacillaceae
Carboxylic ester hydrolases
Characterization
Bacillales
Gene
Bacteria
Hydrolases
Isolation
Gluconolactonase
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Summary:An AHL lactonase gene (aiiA) was PCR amplified from the genomic DNA of Bacillus amyloliquefaciens, with the intact open reading frame of 753 base pair. The gene shares high identity to its homologues present in different Bacillus species. The expression plasmid carrying a tact aiiA-PEBA gene was constructed and the gene was overproduced in Escherichia coli BL21 (DE3). The product expressed resulted in attenuation and suspension of the infection of Pectobacterium carotovorum subsp. carotovorum on carrot. This study verified the existence of the aiiA gene in B. amyloliquefaciens and provided a prospect of the strain as biocontrol agents with quorum quenching property on bacterial disease control.
Bibliography:http://dx.doi.org/10.1007/s11274-010-0308-8
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ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-010-0308-8