Simultaneous purification of the neuroproteins synapsin I and synaptophysin

A procedure for the simultaneous purification of synapsin I and synaptophysin from calf brain was developed. Demyelinated membranes were extracted with 2% Triton X-100 and 2 M KCl. The extracted proteins were separated by weak cation-exchange chromatography on carboxymethyl-Sepharose Fast Flow. Syna...

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Bibliographic Details
Published inJournal of chromatography Vol. 596; no. 1; p. 51
Main Authors Llona, I, Annaert, W G, De Potter, W P
Format Journal Article
LanguageEnglish
Published Netherlands 03.04.1992
Subjects
Animals
Brain Chemistry
Cattle
Chromatography, Affinity
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Octoxynol
Polyethylene Glycols
Potassium Chloride
Synapsins - isolation & purification
Synaptophysin - isolation & purification
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Summary:A procedure for the simultaneous purification of synapsin I and synaptophysin from calf brain was developed. Demyelinated membranes were extracted with 2% Triton X-100 and 2 M KCl. The extracted proteins were separated by weak cation-exchange chromatography on carboxymethyl-Sepharose Fast Flow. Synaptophysin was finally purified by preparative sodium dodecyl sulphate-polyacrylamine gel electrophoresis and synapsin I by affinity chromatography using a calmodulin-Sepharose column. The recovery obtained was 40 micrograms/g in brain for synaptophysin and 25 micrograms/g in brain for synapsin I.
DOI:10.1016/0021-9673(92)80201-5