Gene delivery using cell penetrating peptides-zeolitic imidazolate frameworks

• Published in: Microporous and mesoporous materials Vol. 300; p. 110173
• Main Authors: Abdelhamid, Hani Nasser, Dowaidar, Moataz, Hällbrink, Mattias, Langel, Ülo
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 15.06.2020
• Subjects: Cell-penetrating peptides; Gene delivery; Hierarchical porous materials; Metal-organic frameworks; Zeolitic imidazolate frameworks; Hierarchical porous materials; Gene delivery; Zeolitic imidazolate frameworks; Cell-penetrating peptides; Metal-organic frameworks
• ISSN: 1387-1811; 1873-3093; 1873-3093
• DOI: 10.1016/j.micromeso.2020.110173

Cell-penetrating peptides (CPPs), and metal-organic frameworks (MOFs) are promising as next-generation for the delivery of gene-based therapeutic agents. Oligonucleotide (ON)-mediated assembly of nanostructures composed of hierarchical porous zeolitic imidazolate framework (ZIF-8), and nanoparticles such as graphene oxide (GO), and magnetic nanoparticles (MNPs) for gene therapy are reported. Five different types of non-viral vectors (ZIF-8, RhB@ZIF-8, BSA@ZIF-8, MNPs@ZIF-8, and GO@ZIF-8), and three gene therapeutic agents (plasmid, splice correction oligonucleotides (SCO), and small interfering RNA (siRNA)) were investigated. The polyplexes were characterized and applied for gene transfection. The materials show very low toxicity with high efficiency for luciferase transfection. ZIF-8 enhances the transfection of plasmid, SCO, siRNA of CPPs by 2–8 folds. The mechanism of the cell uptakes was also highlighted. Data reveal cell internalization via scavenger class A (SCARA). [Display omitted] •Synthesis of hierarchical porous zeolitic imidazolate frameworks.•Synthesis of ZIF-8 composite with nanoparticles.•Investigate ZIF-8 and their composite for gene delivery.•Explore reasonable mechanism of cell uptake for oligonucleotides@ZIF-8.