Micromonospora fluostatini sp. nov., isolated from marine sediment

• Published in: International journal of systematic and evolutionary microbiology Vol. 65; no. 12; pp. 4417 - 4423
• Main Authors: Phongsopitanun, Wongsakorn, Kudo, Takuji, Mori, Mihoko, Shiomi, Kazuro, Pittayakhajonwut, Pattama, Suwanborirux, Khanit, Tanasupawat, Somboon
• Format: Journal Article
• Language: English
• Published: England 01.12.2015
• Subjects: Bacterial Typing Techniques; Base Composition; Diaminopimelic Acid - chemistry; DNA, Bacterial - genetics; Fatty Acids - chemistry; Geologic Sediments - microbiology; Micromonospora - classification; Micromonospora - genetics; Micromonospora - isolation & purification; Molecular Sequence Data; Peptidoglycan - chemistry; Phospholipids - chemistry; Phylogeny; Pigmentation; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Thailand; Vitamin K 2 - chemistry
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.000589

The novel actinomycete strain PWB-003T, which produced fluostatins B and C antibiotics, was isolated from nearshore sediment collected from Panwa Cape, Phuket Province, Thailand. Data from the present polyphasic study indicated that strain PWB-003T represented a member of the genus Micromonospora. It produced single spores on substrate mycelia and contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Whole-cell hydrolysate contained ribose, xylose, arabinose, mannose and glucose. The predominant menaquinone was MK-10 (H4). Cellular fatty acids comprised C18 : 1ω9c, iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0. On the basis of 16S rRNA gene sequence similarity analysis, the novel strain was closely related to Micromonospora eburnea LK2-10T (99.38 %), Micromonospora chaiyaphumensis MC5-1T (99.16 %), Micromonospora yangpuensis FXJ6.011T (98.97 %), Micromonospora echinaurantiaca DSM 43904T (98.97 %), Micromonospora pallida DSM 43817T (98.97 %), Micromonospora sagamiensis DSM 43912T and Micromonospora auratinigra JCM 12357T (both 98.97 %). The G+C content of the DNA was 74.5 mol%. DNA-DNA relatedness values among strain PWB-003T and related type strains ranged from 11.3 ± 1.3 to 38.8 ± 1.1 %. On the basis of these observations, strain PWB-003T could be distinguished from its closely related type strains and is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora fluostatini sp. nov. (type strain PWB-003T = JCM 30529T = PCU 341T = TISTR 2345T) is proposed.