Enhanced biocatalysis mechanism under microwave irradiation in isoquercitrin production revealed by circular dichroism and surface plasmon resonance spectroscopy
[Display omitted] •Microwave irradiation was firstly used in enzymatic synthesis of isoquercitrin.•Reaction time (10min) was greatly decreased to one sixty than that in a batch reactor.•Vm/Km under microwave irradiation was 17.49-fold higher than that in a batch reactor.•Secondary structure data of...
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Published in | Bioresource technology Vol. 205; pp. 48 - 57 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.04.2016
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Subjects | |
Online Access | Get full text |
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Summary: | [Display omitted]
•Microwave irradiation was firstly used in enzymatic synthesis of isoquercitrin.•Reaction time (10min) was greatly decreased to one sixty than that in a batch reactor.•Vm/Km under microwave irradiation was 17.49-fold higher than that in a batch reactor.•Secondary structure data of hesperidinase proved the higher affinity to rutin by CD.•SPR showed rutin had a higher affinity to hesperidinase under microwave irradiation.
An efficient and rapid process for isoquercitrin production by hesperidinase-catalyzed hydrolysis of rutin was successfully developed under microwave irradiation detecting the affinity by circular dichroism (CD) and surface plasmon resonance (SPR) spectroscopy. A maximum isoquercitrin yield of 91.5±2.7% was obtained in 10min with the conditions of 10g/L hesperidinase, 2g/L rutin, 30°C and microwave power density 88.9W/L. Enzymatic reaction rate and Vm/Km in the microwave reactor were 6.34-fold higher than in a continuous flow microreactor and 1.24-fold higher than in a biphasic system. CD and SPR analysis results also showed that hesperidinase has a better selectivity and affinity (3.3-fold than in a batch reactor) to generate isoquercitrin under microwave irradiation. Microwave irradiation greatly improved the reaction efficiency and productivity, leading to a more positive economical assessment. The binding affinity indicates the presence of strong multivalent interactions between rutin and hesperidinase under microwave irradiation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0960-8524 1873-2976 |
DOI: | 10.1016/j.biortech.2016.01.009 |