Effect of short pulsed nonablative infrared laser irradiation on vascular cells in vitro and neointimal hyperplasia in a rabbit balloon injury model

• Published in: Circulation (New York, N.Y.) Vol. 104; no. 15; pp. 1850 - 1855
• Main Authors: KIPSHIDZE, Nicholas, NIKOLAYCHIK, Victor, SAHOTA, Harry, LEON, Martin B, ROUBIN, Gary, MOSES, Jeffrey W, MUCKERHEIDI, Michael, KEELAN, Michael H, CHEKANOV, Valeri, MATERNOWSKI, Michelle, CHAWLA, Paramjit, HERNANDEZ, Irina, IYER, Sriram, DANGAS, George
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 09.10.2001
• Subjects: Angioplasty, Balloon, Coronary - adverse effects; Animals; Biological and medical sciences; Catheterization - instrumentation; Cell Count; Cell Division - radiation effects; Cell Survival - radiation effects; Cells, Cultured; Disease Models, Animal; Diseases of the cardiovascular system; Dose-Response Relationship, Radiation; Endothelium, Vascular - cytology; Endothelium, Vascular - radiation effects; Fiber Optic Technology - instrumentation; Hyperplasia - etiology; Hyperplasia - pathology; Hyperplasia - prevention & control; Iliac Artery - pathology; Iliac Artery - radiation effects; Iliac Artery - surgery; Infrared Rays - therapeutic use; Laser Therapy; Medical sciences; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - radiation effects; Rabbits; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Treatment Outcome; Tunica Intima - injuries; Tunica Intima - radiation effects; Cell culture; Endothelial cell; Hyperplasia; Rabbit; Cuffed tube; Cardiovascular disease; Supplemented diet; Myocyte; Vascular disease; Prevention; Intima; Complication; Vascular wall; Treatment efficiency; Instrumentation therapy; Instrumental dilatation; Lagomorpha; Iliac artery; Cholesterol; Restenosis; Vertebrata; Concomitant disease; Mammalia; Treatment; Infrared laser; Animal; Lesion
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/hc3901.096101

Neointimal hyperplasia after PTCA is an important component of restenosis. Cultures of rabbit endothelial cells and smooth muscle cells (SMCs) were irradiated with different doses of nonablative infrared (1064-nm) radiation. Normalized viability index detected with nondestructive Alamar Blue assay and direct cell count were studied. Our experiments demonstrated dose-dependent cytostatic or cytotoxic effects of laser irradiation. We also evaluated the long-term effect of endoluminal nonablative infrared laser irradiation on neointimal hyperplasia in a rabbit balloon injury model. PTCA of both iliac arteries of 23 New Zealand White rabbits was performed. One iliac artery was subjected to intra-arterial subablative infrared irradiation via a diffuse tip fiber. The contralateral vessel served as control. The diet was supplemented with 0.25% cholesterol and 2% peanut oil for 10 days before and 60 days after PTCA. Morphometry after 60 days showed that intimal areas were 0.76+/-0.18 and 1.85+/-0.30 mm(2) in the laser and control arteries, respectively (P=2.2x10(-11)). We conclude that nonablative infrared laser inhibited neointimal hyperplasia after PTCA in cholesterol-fed rabbits for up to 60 days.