Actinomadura montaniterrae sp. nov., isolated from mountain soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 9; pp. 3310 - 3316
• Main Authors: Songsumanus, Apakorn, Kudo, Takuji, Ohkuma, Moriya, Phongsopitanun, Wongsakorn, Tanasupawat, Somboon
• Format: Journal Article
• Language: English
• Published: England 01.09.2016
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Bacterial Typing Techniques; Base Composition; Cell Wall - chemistry; Diaminopimelic Acid - chemistry; DNA, Bacterial - genetics; Fatty Acids - chemistry; Nucleic Acid Hybridization; Peptidoglycan - chemistry; Phospholipids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Thailand; Vitamin K 2 - chemistry
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.001196

The taxonomic position of the mountain soil actinomycete, strain CYP1-1BT, was clarified by a polyphasic study. The strain produced a single spore, or occasionally a chain of spores, on aerial mycelium. Chemotaxonomic data supported the classification of CYP1-1BT as representing a member of the genus Actinomadura on the basis of the presence of meso-diaminopimelic acid in the peptidoglycan; galactose, glucose, madurose and ribose as whole cell sugars; MK-9(H6), MK-9(H8) and MK-9(H4) as dominant menaquinones; C16 : 0, 10-methylated C18 : 0 and C18 : 1ω9c as the major cellular fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol manosides as the predominant phospholipids. The DNA G+C content was 74.3 mol%. On the basis of the combination of morphological and chemotaxonomic characteristics, CYP1-1BT was identified as representing a member of the genus Actinomadura. On the basis of the results of 16S rRNA gene analysis, CYP1-1BT, was shown to be closely related to Actinomadura nitritigenes DSM 44137T (98.9 %). Phenotypic, genotypic and DNA-DNA hybridization data supported the hypothesis that CYP1-1BT represents a novel species of the genus Actinomadura for which the name Actinomadura montaniterrae sp. nov. is proposed. The type strain is CYP1-1BT (=JCM 16995T=KCTC 39784T=PCU 349T=TISTR 2400T).