Evidence for Alternative Splicing of a Dopamine D2 Receptor in a Teleost

• Published in: Physiological and biochemical zoology Vol. 84; no. 2; pp. 135 - 146
• Main Authors: Popesku, Jason T., Navarro-Martín, Laia, Trudeau, Vance L.
• Format: Journal Article
• Language: English
• Published: United States University of Chicago Press 01.03.2011
• Subjects: Alternative Splicing - physiology; Amino Acid Sequence; Amino acids; Animals; Base Sequence; Brain - physiology; Carassius auratus; Cloning, Molecular; Complementary DNA; DNA - genetics; Exons; Freshwater; Genome; Goldfish; Goldfish - metabolism; Introns; Molecular Sequence Data; Pituitary Gland - physiology; Polymerase chain reaction; Polymerase Chain Reaction - methods; Protein Isoforms; Receptors; Receptors, Dopamine D2 - genetics; Receptors, Dopamine D2 - metabolism; Seasons; Splicing; Teleostei; Tissue Distribution; Vertebrates
• ISSN: 1522-2152; 1537-5293
• DOI: 10.1086/658290

Previous attempts at identifying an alternatively spliced dopamine (DA) D2 receptor in teleosts have proven unsuccessful. We provide evidence of a splicing event of a goldfish D2 (gfD2b1) receptor in the neuroendocrine brain of adult goldfish that produces a spliced short isoform (gfD2b1S). We also identify an additional novel D2b paralog (gfD2b2) that does not appear to be alternatively spliced in adult fish during the reproductive cycle. Relatively high mRNA levels of gfD2b1S were observed in the neuroendocrine brain and pituitary of sexually immature fish compared with sexually regressing fish. Real-time RT-PCR revealed that intraperitoneal injection of either SCH 23390 or sulpiride—D1- or D2-specific antagonists, respectively—decreased mRNA levels of gfD2b1S by 3.9-fold without affecting the unspliced isoforms. We suggest that the expression of the spliced D2 receptor modulates the inhibitory tone of DA throughout the reproductive cycle. The deduced amino acid sequence of gfD2b1S lacks 29 amino acids in the same region as the short isoform of mammalian D2. We propose that the gfD2b1S splice variant is the teleost ortholog of mammalian D2S. The hypothesis that D2 receptor splicing is a relatively recent innovation in higher tetrapods is not supported by our results.