Characterization of Acinetobacter indicus ZJB20129 for heterotrophic nitrification and aerobic denitrification isolated from an urban sewage treatment plant

• Published in: Bioresource technology Vol. 347; p. 126423
• Main Authors: Ke, Xia, Liu, Cong, Tang, Su-Qin, Guo, Ting-Ting, Pan, Li, Xue, Ya-Ping, Zheng, Yu-Guo
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2022
• Subjects: Acinetobacter; Aerobic denitrification; Aerobiosis; ammonium; Ammonium Compounds; ammonium nitrogen; biochemical pathways; Biological treatment; carbon; Denitrification; Heterotrophic nitrification; Heterotrophic Processes; hydroxylamine; nitrate nitrogen; nitrate reductase; Nitrification; nitrite nitrogen; nitrite reductase; Nitrites; Nitrogen; Nitrogen removal functional enzyme; Sewage; sewage treatment; sodium; succinic acid; temperature; wastewater; Heterotrophic nitrification; Biological treatment; Aerobic denitrification; Nitrogen removal functional enzyme
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2021.126423

[Display omitted] •A novel HN-AD Acinetobacter sp. was isolated with excellent nitrogen removal capability.•It tolerated high inorganic nitrogen (100 mg/L) and exhibited rapid removal rate.•It could effectually remove NH4+ in simultaneous nitrification and denitrification.•Proposed metabolic pathway of HN-AD was predicted after enzyme identification. The Acinetobacter indicus strain ZJB20129 isolated from an urban sewage treatment plant demonstrated the heterotrophic nitrification-aerobic denitrification (HN-AD) ability. Strain ZJB20129 could remove 98.73% of ammonium-N, 97.26% of nitrite-N and 96.55% of nitrate-N, and the maximum removal rate was 3.66, 4.62 and 5.21 mg/L/h, respectively. Ammonium was preferentially used during simultaneous nitrification and denitrification. Strain ZJB20129 exhibited highest ammonium removal capability when carbon source was sodium succinate, C/N ratio was 15, pH was 8.0, and temperature was 35 ℃. Key enzymes involved in HN-AD including hydroxylamine oxidase, periplasmic nitrate reductase and nitrite reductase as well as their encoding genes were detected, and the metabolic pathway of HN-AD was subsequently predicted. Our results suggested that Acinetobacter indicus ZJB20129 displayed superior nitrogen removal performance on actual wastewater and thus made it have a good application prospect in wastewater biological treatment.