Helicobacter species sequences in liver samples from patients with and without hepatocellular carcinoma

• Published in: World journal of gastroenterology : WJG Vol. 10; no. 4; pp. 598 - 601
• Main Authors: Pellicano, Rinaldo, Mazzaferro, Vincenzo, Grigioni, Walter Franco, Cutufia, Miguel Angel, Fagoonee, Sharmila, Silengo, Lorenzo, Rizzetto, Mario, Ponzetto, Antonio
• Format: Journal Article
• Language: English
• Published: United States Department of Gastro-Hepatology, Ospedale S. Giovanni Battista (Molinette),Torino, Italy%Surgery and Liver Transplantation Centre,Istituto Nazionalc dei Tumori, Milano, Italy%Department of Pathology, Istituto Addarii,Bologna, Italy%Department of Biology, Biochemistry and Genetics, University of Torino, Itlay%Department of Biology, Biochemistry and Genetics, University of Torino, Italy 15.02.2004; Baishideng Publishing Group Inc
• Subjects: Brief Reports; Carcinoma, Hepatocellular - microbiology; Carcinoma, Hepatocellular - pathology; Colonic Neoplasms - secondary; DNA, Bacterial - analysis; DNA序列分析; Helicobacter Infections - complications; Helicobacter Infections - microbiology; Helicobacter Infections - pathology; Helicobacter pylori - genetics; Helicobacter pylori - isolation & purification; Humans; Liver Cirrhosis - microbiology; Liver Cirrhosis - pathology; Liver Neoplasms - microbiology; Liver Neoplasms - pathology; 乙型肝炎病毒; 幽门螺杆菌; 肝组织; 肝细胞癌
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v10.i4.598

AIM:Only a minority of patients carrying a defined viral aetiologic agent develop cirrhosis and ultimately hepatocellular carcinoma (HCC), the mechanism underlying the worsening is still undefined. Experimental infection by Helicobacter hepaticusin mice causes chronic hepatitis and HCC and recently, more Helicobacter species (Helicobacter spp.) have been detected in the liver of patients suffering from cholestatic diseases and HCC arising from non-cirrhotic liver. We investigated whether Helicobacter spp. sequences could be detected in the liver of patients with cirrhosis and HCC compared to subjects with metastasis to liver from colon cancer.METHODS:Twenty-three liver samples from patients operated upon for HCC superimposed on hepatitis C virus (HCV)-related cirrhosis and 6 from patients with resected metastases from colorectal cancer, were tested by polymerase chain reaction for presence of genomic 16S rRNA of Helicobacter genus using specific primers. DNA sequencing and cagA gene analysis were also performed.RESULTS:Genomic sequences of Helicobacter spp. were found in 17 of 20 (85%) liver samples from patients with HCC and in 2 of 6 samples from patients with liver metastasis.In three samples of the first group the result was uncertain.Hpyloriwas revealed in 16 out of 17 positive samples and Helicobacter pullorum in the other.CONCLUSION: Helicobacter spp., carcinogenic in mice,were found at a higher frequency in the liver of patients with HCV-related cirrhosis and HCC than those in patients without primary liver disease.