Development of murine monoclonal antibodies against an enterotoxin produced by Bacillus cereus

• Published in: Journal of Veterinary Medical Science Vol. 53; no. 3; pp. 419 - 422
• Main Authors: Shinagawa, K. (Iwate Univ., Morioka (Japan). Faculty of Agriculture), Yokoi, R, Matsusaka, N, Sugii, S
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.06.1991
• Subjects: Animals; Antibodies, Bacterial - biosynthesis; Antibodies, Bacterial - immunology; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Antibody Specificity; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; BACILLUS CEREUS; Blotting, Western; CULTURE TECHNIQUES; enterotoxin; ENTEROTOXINAS; ENTEROTOXINE; ENTEROTOXINS; Enterotoxins - immunology; Enzyme-Linked Immunosorbent Assay; Foodborne Diseases - microbiology; HIBRIDOMA; HYBRIDOMAS; HYBRIDOME; ISOLATION TECHNIQUES; Male; MICE; Mice, Inbred BALB C; MONOCLONAL ANTIBODIES; monoclonal antibody; PURIFICACION; PURIFICATION; RATON; SOURIS; TECHNIQUE DE CULTURE; TECHNIQUE DE L'ISOLEMENT; TECNICAS DE AISLAMIENTO; TECNICAS DE CULTIVO
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.53.419

Three murine monoclonal antibodies (MAbs) were prepared against an enterotoxin (ET) produced by Bacillus cereus. Although these MAbs were found to react with the ET, their specificities appeared to be different in competitive binding assays. One of the MAbs (D-8), which was highly reactive with the ET, will be useful in developing immunological methods to detect crude ET and to isolate the ET in high yield.