Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1® Advanced XL System

• Published in: Methods and protocols Vol. 8; no. 1; p. 2
• Main Authors: Neilson, Scarlet, Nangeroni, Leah, Ghemrawi, Mirna
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.01.2025; MDPI
• Subjects: Analysis; Antigens; Automation; body fluid identification; body fluid mixtures; Deoxyribonucleic acid; differential DNA extraction; DNA; Ethylenediaminetetraacetic acid; Forensic science; Invoices; lateral flow immunochromatographic (LFI) tests; Menstruation; Protocol; Sex crimes; Sexual abuse; Sperm; Vortices; United States; Massachusetts; Germany; Pennsylvania; United States > US; differential DNA extraction; body fluid identification; body fluid mixtures; lateral flow immunochromatographic (LFI) tests

This differential extraction protocol details the steps for isolating DNA from sample pads used in lateral flow immunochromatographic (LFI) tests, particularly for cases involving mixed biological samples such as semen and menstrual blood, or other evidence related to sexual assault. This procedure...