Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1® Advanced XL System

• Published in: Methods and protocols Vol. 8; no. 1; p. 2
• Main Authors: Neilson, Scarlet, Nangeroni, Leah, Ghemrawi, Mirna
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.01.2025; MDPI
• Subjects: Analysis; Antigens; Automation; body fluid identification; body fluid mixtures; Deoxyribonucleic acid; differential DNA extraction; DNA; Ethylenediaminetetraacetic acid; Forensic science; Invoices; lateral flow immunochromatographic (LFI) tests; Menstruation; Protocol; Sex crimes; Sexual abuse; Sperm; Vortices; United States; Massachusetts; Germany; Pennsylvania; United States > US; differential DNA extraction; body fluid identification; body fluid mixtures; lateral flow immunochromatographic (LFI) tests
• ISSN: 2409-9279; 2409-9279
• DOI: 10.3390/mps8010002

This differential extraction protocol details the steps for isolating DNA from sample pads used in lateral flow immunochromatographic (LFI) tests, particularly for cases involving mixed biological samples such as semen and menstrual blood, or other evidence related to sexual assault. This procedure utilizes a differential extraction technique applied to sample pads from immunochromatographic tests, where the sample pads serve as the substrate. The method involves two sequential lysis steps to effectively separate non-sperm and sperm fractions, enabling the targeted isolation of distinct cell types for downstream DNA analysis. The efficiency of this procedure is demonstrated by the results within this paper, which highlights the successful recovery of both male autosomal and Y-STR profiles, even in mixed samples with a high female presence. Overall, this protocol demonstrates the effective recovery of DNA from sample pads, which is beneficial for forensic practitioners dealing with limited sample quantities, underscoring the value of using these pads in forensic analysis.