Factors affecting short-term and long-term stabilities of proteins

Proteins are marginally stable and hence readily denatured by various stresses encountered in solution, or in the frozen or dried states. Various additives are known to minimize the damage and to enhance the stability of proteins. This review discusses the current knowledge of the mechanisms by whic...

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Bibliographic Details
Published inAdvanced drug delivery reviews Vol. 10; no. 1; pp. 1 - 28
Main Authors Arakawa, Tsutomu, Prestrelski, Steven J., Kenney, William C., Carpenter, John F.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 1993
Subjects
Co-solute induced stabilization of proteins
Cryoprotectant
Freeze-induced denaturation
Lyophilization-induced denaturation
Protein degradation
Protein stabilization
FTIR, Fourier transform infrared
Protein degradation
Co-solute induced stabilization of proteins
PEG, polyethylene glycol
MPD, 2-methyl-2,4-pentanediol
PFK, phosphofructokinase
G-CSF, granulocyte-colony stimulating factor
Cryoprotectant
Lyophilization-induced denaturation
FGF, fibroblast growth factor
Freeze-induced denaturation
Protein stabilization
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Summary:Proteins are marginally stable and hence readily denatured by various stresses encountered in solution, or in the frozen or dried states. Various additives are known to minimize the damage and to enhance the stability of proteins. This review discusses the current knowledge of the mechanisms by which these additives stabilize proteins against acute stresses, and also the various factors to be considered for long-term storage of proteins in solution.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0169-409X
1872-8294
DOI:10.1016/0169-409X(93)90003-M