The effects of tritium on embryo development: the embryotoxic effects of [3H]tryptophan

The effects of tritiated amino acids on the development of the early mouse embryo in vitro are described. [3H]tryptophan was extremely toxic, compared with [3H]glutamic acid, and inhibited growth of the two-cell embryo to the blastocyst stage. The LD50 values for the two amino acids were, respective...

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Bibliographic Details
Published inInternational journal of radiation biology Vol. 56; no. 2; p. 139
Main Authors Killen, H M, Carroll, J
Format Journal Article
LanguageEnglish
Published England 1989
Subjects
Age Factors
Animals
Blastocyst - radiation effects
Cell Compartmentation
Chromosomes - metabolism
Cleavage Stage, Ovum - radiation effects
Embryo Implantation
Female
Glutamates - metabolism
Glutamic Acid
In Vitro Techniques
Leucine - metabolism
Mice - embryology
Molecular Weight
Nuclear Proteins - biosynthesis
Protein Biosynthesis
RNA, Messenger - metabolism
Tritium - toxicity
Tryptophan - metabolism
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Summary:The effects of tritiated amino acids on the development of the early mouse embryo in vitro are described. [3H]tryptophan was extremely toxic, compared with [3H]glutamic acid, and inhibited growth of the two-cell embryo to the blastocyst stage. The LD50 values for the two amino acids were, respectively, 1 and 296 kBq/ml of medium. The two-cell embryo was more sensitive to the irradiation from incorporated [3H]tryptophan is selectively incorporated into two chromosomal proteins in the two-cell embryo, and that those proteins were stage-specific markers of development.
ISSN:0955-3002
DOI:10.1080/09553008914551291