Physicochemical evaluation, structural characterization, in vitro and in vivo bioactivities of water-soluble polysaccharides from Luobuma (Apocynum L.) tea

• Published in: Food chemistry Vol. 460; no. Pt 2; p. 140453
• Main Authors: Abubakar, Aminu Shehu, Ahmad, Bilal, Ahmad, Nabi, Liu, Liangliang, Liu, Bin, Qu, Yatong, Chen, Jikang, Chen, Ping, Zhao, Haohan, Chen, Jia, Chen, Kunmei, Gao, Gang, Zhu, Aiguo
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2024
• Subjects: Animals; Anti-inflammatory; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Apocynum; Apocynum sp; arabinose; carrageenan; Carrageenan-induced edema; China; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; dose response; edema; Edema - chemically induced; Edema - drug therapy; food chemistry; fucose; galactose; glucose; herbal tea; Interleukin-6; Interleukin-6 - genetics; Interleukin-6 - immunology; Interleukin-6 - metabolism; Japan; Luobuma tea; macrophages; Macrophages - drug effects; Macrophages - immunology; Male; mannose; Mice; Molecular Weight; necrosis; neoplasms; Nitric oxide; Nitric Oxide - metabolism; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; prostaglandin synthase; RAW 264.7 Cells; rhamnose; species; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology; Tumor Necrosis Factor-alpha - metabolism; water solubility; xylose; China; Apocynum sp; Antioxidants; Polysaccharides; Nitric oxide; Luobuma tea; Carrageenan-induced edema; Anti-inflammatory; Interleukin-6
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2024.140453

Luobuma tea is made from the leaves of Apocynum hendersonii (Bt) and A. venetum (Ht) and has been used for a very long time in China and Japan as herbal tea. This study isolated water-soluble polysaccharides from the two species` teas. Physicochemical properties, structural properties, in vitro and in vivo antioxidant and immunomodulatory activities were determined for the first time. The results showed that the Bt and Ht polysaccharides with molecular weights of 31.21 and 49.11 kDa, respectively, composed of arabinose, galactose, rhamnose, glucose, xylose, fucose, and mannose. A dose-dependent nitric oxide production and interleukin-6 inhibitory effects were obtained. Also, they suppressed the expression of cyclooxygenase-2, tumor necrosis factor-α and interleukin-6 mRNA in LPS-induced RAW 264.7 macrophages. Likewise, Bt and Ht have significantly reduced edema in the paws of mice after carrageenan injection. These results suggested that the Luobuma teas polysaccharides can be explored as potential antioxidants and anti-inflammatory agents. •A soluble polysaccharides (Bt and Ht) were extracted from Luobuma (Apocynum L.) tea.•Physico-chemical and structural properties of the Bt and Ht were investigated.•Bt and Ht have inhibited the production of interleukin-6 inflammatory mediator in macrophages.•Bt and Ht have significantly reduced edema in the paws of mice after carrageenan injection.