Characterization and mRNA expression profile of the TbNre1 gene of the ectomycorrhizal fungus Tuber borchii
This study focuses on the cloning and characterization of the major nitrogen regulator element from the ectomycorrhizal fungus Tuber borchii, TbNre1 . Sequence analysis of the predicted protein and complementation experiments in Neurospora crassa demonstrated that the cloned gene is orthologous to a...
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Published in | Current genetics Vol. 55; no. 1; pp. 59 - 68 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer-Verlag
01.02.2009
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | This study focuses on the cloning and characterization of the major nitrogen regulator element from the ectomycorrhizal fungus
Tuber borchii, TbNre1
. Sequence analysis of the predicted protein and complementation experiments in
Neurospora crassa
demonstrated that the cloned gene is orthologous to
areA/nit-2
gene. Transcriptional expression investigations by real-time RT-PCR showed
TbNre1
up-regulation in the presence of nitrate or in the absence of nitrogen during free-living mycelium growth. On the contrary,
TbNre1
mRNA levels remained at basal values in the presence of preferred nitrogen sources like ammonium and glutamine. Furthermore,
TbNre1
mRNA was found to be up-regulated during
T. borchii
and
T. platyphyllos
interaction. All these data suggest that the regulatory protein TBNRE1 could play a major role in regulating N metabolism genes of
T. borchii
in the free living mycelium and in
T. borchii
–
T. platyphyllos
interaction. Finally, the possible role of the transcription factor
TBNRE1
in the induction of proteases and virulence-like genes, necessary in ectomycorrhizal establishment, was also discussed. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0172-8083 1432-0983 |
DOI: | 10.1007/s00294-008-0222-x |