Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125

• Published in: Chinese journal of chemical engineering Vol. 18; no. 5; pp. 870 - 875
• Main Author: 李政 邓利 鲁吉珂 郭小雷 杨自信 谭天伟
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.10.2010
• Subjects: Candida; Crude oil; Esters; Fatty acids; immobilized lipase; Lipase; methanolysis; Methyl alcohol; optimization; Oryza sativa; Rice bran oil; Solvents; transesterification; 假丝酵母; 固定化脂肪酶; 最佳条件; 生物柴油; 米糠油; 脂肪酸甲酯; 酶法合成; rice bran oil; methanolysis; optimization; transesterification; immobilized lipase
• ISSN: 1004-9541; 2210-321X
• DOI: 10.1016/S1004-9541(09)60141-5

The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were： 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 °C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.