Mitochondrial alternative oxidase is involved in both compatible and incompatible host-virus combinations in Nicotiana benthamiana

• Published in: Plant science (Limerick) Vol. 239; pp. 26 - 35
• Main Authors: Zhu, Feng, Deng, Xing-Guang, Xu, Fei, Jian, Wei, Peng, Xing-Ji, Zhu, Tong, Xi, De-Hui, Lin, Hong-Hui
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.10.2015
• Subjects: Alternative oxidase (AOX); biotic stress; cyanide-resistant respiration; death; Disease Resistance; Gene Expression Regulation, Plant; Gene Silencing; genes; genetic markers; hypersensitive response; leaves; messenger RNA; mitochondria; Mitochondrial Proteins - genetics; Mitochondrial Proteins - metabolism; Molecular Sequence Data; Nicotiana - genetics; Nicotiana - immunology; Nicotiana - metabolism; Nicotiana benthamiana; Oxidoreductases - genetics; Oxidoreductases - metabolism; Plant Diseases - genetics; Plant Diseases - virology; Plant Immunity; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - immunology; Plants, Genetically Modified - metabolism; potassium cyanide; Sequence Alignment; Sequence Analysis, Protein; Systemic resistance; Tobacco mosaic virus; Tobacco mosaic virus (TMV); Tobacco Mosaic Virus - physiology; Turnip crinkle virus (TCV); Virus-induced gene-silencing (VIGS); viruses; Turnip crinkle virus (TCV); Virus-induced gene-silencing (VIGS); Alternative oxidase (AOX); Systemic resistance; Tobacco mosaic virus (TMV)
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2015.07.009

•We studied the mechanisms of AOX in the systemic antiviral defense response.•We used VIGS to investigate the role of AOX in N-mediated resistance to TMV.•NbAOX plays an important role in the systemic resistance to virus infection.•NbAOX is required for N-mediated resistance to TMV. The alternative oxidase (AOX) functions in the resistance to biotic stress. However, the mechanisms of AOX in the systemic antiviral defense response and N (a typical resistance gene)-mediated resistance to Tobacco mosaic virus (TMV) are elusive. A chemical approach was undertaken to investigate the role of NbAOX in the systemic resistance to RNA viruses. Furthermore, we used a virus-induced gene-silencing (VIGS)-based genetics approach to investigate the function of AOX in the N-mediated resistance to TMV. The inoculation of virus significantly increased the NbAOX transcript and protein levels and the cyanide-resistant respiration in the upper un-inoculated leaves. Pretreatment with potassium cyanide greatly increased the plant's systemic resistance, whereas the application of salicylhydroxamic acid significantly compromised the plant's systemic resistance. Additionally, in NbAOX1a-silenced N-transgenic Nicotiana benthamiana plants, the inoculated leaf collapsed and the movement of TMV into the systemic tissue eventually led to the spreading of HR-PCD and the death of the whole plant. The hypersensitive response marker gene HIN1 was significantly increased in the NbAOX1a-silenced plants. Significant amounts of TMV-CP mRNA and protein were detected in the NbAOX1a-silenced plants but not in the control plants. Overall, evidence is provided that AOX plays important roles in both compatible and incompatible plant-virus combinations.