Characterization of Camptotheca acuminata 10-hydroxygeraniol oxidoreductase and iridoid synthase and their application in biological preparation of nepetalactol in Escherichia coli featuring NADP+ - NADPH cofactors recycling

• Published in: International journal of biological macromolecules Vol. 162; pp. 1076 - 1085
• Main Authors: Awadasseid, Annoor, Li, Wei, Liu, Zhan, Qiao, Chong, Pang, Jing, Zhang, Guolin, Luo, Yinggang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2020
• Subjects: 10-Hydroxygeraniol oxidoreductase; Aphidoidea; biosynthesis; biotransformation; Bridged Bicyclo Compounds, Heterocyclic - metabolism; Camptotheca - enzymology; Camptotheca - genetics; Camptotheca acuminata; Camptothecin; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; fermentation; indoles; Iridoid; Iridoid synthase; iridoids; monoterpenoids; NADP (coenzyme); NADP - metabolism; Nepetalactol; oxidation; Oxidoreductases - biosynthesis; Oxidoreductases - genetics; Plant Proteins - biosynthesis; Plant Proteins - genetics; Iridoid; Camptothecin; 10-Hydroxygeraniol oxidoreductase; Iridoid synthase; Nepetalactol
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.06.223

Nepetalactol, an iridoid with four chiral carbons, is a crucial component of aphid sex pheromones that have been employed with great success to control the insect-related diseases. Despite of agricultural usage as end products, iridoids are fundamental biosynthetic intermediates for pharmaceutically important monoterpenoid indole alkaloids such as camptothecin (CAM) and vinca alkaloids. Herein we characterized 10-hydroxygeraniol oxidoreductase (10HGO) and iridoid synthase (IS) from Camptotheca acuminata, a CAM-producing plant, and reported their application in biological preparation of nepetalactol. Ca10HGO and CaIS were respectively cloned from C. acuminata, overexpressed in Escherichia coli, and purified to homogeneity. Ca10HGO catalyzes the oxidation of 10-hydroxygeraniol into 10-oxogeranial, in which NADP+ was reduced to NADPH. CaIS catalyzes nepetalactol formation from 10-oxogeranial using NADPH cofactor. The net outcome of the two reactions generate nepetalactol from 10-hydroxygeraniol efficiently, indicating NADP+ - NADPH recycling. Ca10HGO and CaIS were co-overexpressed in E. coli under optimized fermentation conditions to prepare cell-based catalysts that catalyze the conversion of 10-hydroxygeraniol into nepetalactol. The present work shows the enzymatic conversion of 10-hydroxygeraniol into nepetalactol involved in CAM biosynthesis. Co-overexpression of Ca10HGO and CaIS in E. coli is an alternative valuable cell-based biotransformation process with regenerating recycling of NADP+ - NADPH cofactors for nepetalactol preparation. •Ca10HGO and CaIS were cloned from camptothecin-producing plant Camptotheca acuminata.•Ca10HGO and CaIS were expressed in Escherichia coli and functionally characterized.•In vitro combinatory assays of Ca10HGO and CaIS verified recycling of NADP+ - NADPH.•10-Hydroxygeraniol was converted into nepetalactol by E. coli expressing Ca10HGO-IS.