Measurement of xylanase activity with insoluble xylan substrate

Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3....

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Published inBiochemical journal Vol. 226; no. 2; pp. 617 - 620
Main Authors Nummi, M, Perrin, J M, Niku-Paavola, M L, Enari, T M
Format Journal Article
LanguageEnglish
Published England 01.03.1985
Subjects
Aspergillus
Aspergillus - enzymology
endo-1,3- beta -D-xylanase
Endo-1,4-beta Xylanases
Glycoside Hydrolases - metabolism
Hydrolysis
nephelometry
Nephelometry and Turbidimetry
Polysaccharides - metabolism
Trichoderma
Trichoderma - enzymology
Xylans - isolation & purification
Xylans - metabolism
Xylosidases - metabolism
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Summary:Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2260617