Arenibacter amylolyticus sp. nov., an amylase-producing bacterium of the family Flavobacteriaceae isolated from marine water in India

• Published in: International journal of systematic and evolutionary microbiology Vol. 71; no. 3
• Main Authors: Sidhu, Chandni, Saini, Mohit Kumar, Srinivas Tanuku, Naga Radha, Pinnaka, Anil Kumar
• Format: Journal Article
• Language: English
• Published: England 01.01.2021
• Subjects: 16S rRNA gene-based phylogeny; Arenibacter amylolyticus; chemotaxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.004664

A novel Gram-stain-negative, curved rod-shaped, 0.5-0.7 µm wide and 3.0-10.0 µm long, non-motile bacterium, designated strain AK53 , was isolated from a 5 m depth water sample collected from the Bay of Bengal, Visakhapatnam, India. Colonies on marine agar were circular, small, dark orange, shiny, smooth, translucent, flat, with an entire margin. The major fatty acids included iso-C , iso-C 3OH, anteiso-C , iso-C G, iso-C 3OH and summed feature 3 (C 7 and/or C 6 and/or iso-C -2OH). Polar lipids included phosphatidylethanolamine and five unidentified lipids. The DNA G+C content of the strain AK53 was found to be 40.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AK53 was closely related to KMM 426 and KMM3941 (pair-wise sequence similarity of 99.17 and 98.89 %, respectively), forming a distinct branch within the genus and clustering with . Strain AK53 shared average nucleotide identity (ANIb, based on blast) of 78.07 and 77.44 % with JCM 13508 and JCM 13507 , respectively. Based on the observed phenotypic, chemotaxonomic characteristics and phylogenetic analysis, strain AK53 is described in this study as representing a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is AK53 (=MTCC 12004 = JCM 19206 =KCTC 62553 ).