Evaluation of the grapevine nursery propagation process as a source of Phaeoacremonium spp. and Phaeomoniella chlamydospora and occurrence of trunk disease pathogens in rootstock mother vines in Spain

• Published in: European journal of plant pathology Vol. 126; no. 2; pp. 165 - 174
• Main Authors: Aroca, Ángeles, Gramaje, David, Armengol, Josep, García-Jiménez, José, Raposo, Rosa
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.02.2010; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Agriculture; Biological and medical sciences; Biomedical and Life Sciences; Botryosphaeria dothidea; Buds; Ecology; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Life Sciences; Nurseries; Pathogens; Peat; Phytopathology. Animal pests. Plant and forest protection; Plant diseases; Plant Pathology; Plant propagation; Plant Sciences; Root development; Wineries & vineyards; Southern Europe; Europe; Spain; spp; Botryosphaeriaceae; Petri disease; Ascomycota; Phomopsis spp; Plant pathology; Plant pathogen; Phomopsis; Vitis vinifera; Fungi; Fruit crop; Vitidaceae; Dicotyledones; Angiospermae; Spermatophyta; Fungi Imperfecti
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-009-9530-3

Five commercial nurseries were sampled in 2007 to evaluate the grapevine nursery propagation process as a source of Petri disease pathogens (Phaeoacremonium spp. and Phaeomoniella chlamydospora). Samples were taken at four stages of the propagation process: pre-grafting hydration tanks, scissors used for cutting buds, grafting machines and peat used to promote root development. All samples were analysed using two different techniques: nested PCR using specific primers for Phaeoacremonium spp. (Pm1/Pm2) and Pa. chlamydospora (Pch1/Pch2); and fungal isolation by culturing on semi-selective medium. Either Phaeoacremonium spp. or Pa. chlamydospora were detected at any of these stages, and more importantly they were viable since they were detected by isolating on culturing medium. Additionally, the importance of grapevine rootstock mother fields as sources of inoculum in the nurseries was studied. Fourteen grapevine rootstock mother fields were surveyed in 2006 and 2007 for the occurrence of fungal trunk pathogens. A total of 16.4% and 30% of the plants sampled in 2006 and 2007, respectively were infected. Petri disease pathogens (Pa. chlamydospora, Phaeoacremonium aleophilum, Pm. parasiticum) and several Botryosphaeriaceae species (Neofusicoccum parvum, Botryosphaeria dothidea, Lasiodiplodia theobromae, N. australe, N. mediterraneum and N. vitifusiforme) and Phomopsis viticola were isolated. This is the first time N. mediterraneum has been isolated from grapevines and the first report of N. australe, N. mediterraneum and N. vitifusiforme in Spain. This work shows that grapevine rootstock mother plants and the propagation process of grapevine plants should be considered as important sources of inoculum for fungal trunk pathogens, and especially of Petri disease pathogens.