Silver nanoparticles biosynthesized from secondary metabolite producing marine actinobacteria and evaluation of their biomedical potential

• Published in: Antonie van Leeuwenhoek Vol. 114; no. 10; pp. 1497 - 1516
• Main Authors: Alam, Ashia, Tanveer, Faouzia, Khalil, Ali Talha, Zohra, Tanzeel, Khamlich, Saleh, Alam, Muhammad Masroor, Salman, Muhammad, Ali, Muhammad, Ikram, Aamer, Shinwari, Zabta Khan, Maaza, Malik
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.10.2021; Springer Nature B.V
• Subjects: Antiinfectives and antibacterials; Antioxidants; Biomedical and Life Sciences; Biosynthesis; Crystallites; Crystals; Electrostatic properties; Fabrication; Functional groups; Gene sequencing; Grain size; Life Sciences; Low concentrations; Mathematical analysis; Medical Microbiology; Metabolites; Microbiology; Nanoparticles; Original Paper; Physicochemical properties; Plant Sciences; Rhodococcus rhodochrous; rRNA 16S; Silver; Soil Science & Conservation; Stabilizers (agents); Streptomyces; Therapeutic applications; Ultrafines; Zeta potential; Antiviral; silver nanoparticles (R-AgNPs); Biomedical applications; Marine actinobacteria; silver nanoparticles (S-AgNPs
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-021-01616-5

Biosynthesis of silver nanoparticles (AgNPs) from marine actinobacteria offers a promising avenue for exploring bacterial extracts as reducing and stabilizing agents. We report extracellular extracts of Rhodococcus rhodochrous (MOSEL-ME29) and Streptomyces sp. (MOSEL-ME28), identified by 16S rRNA gene sequencing for synthesis of AgNPs. Ultrafine silver nanoparticles were biosynthesized using the extracts of R. rhodochrous and Streptomyces sp. and their possible therapeutic applications were studied. The physicochemical properties of nanoparticles were established by HR-SEM/TEM, SAED, UV–Vis, EDS, XRD, and FTIR. UV–Vis spectra displayed characteristic absorption at 430 nm and 412 nm for AgNPs from Streptomyces sp. (S-AgNPs) and Rhodococcus sp. (R-AgNPs), respectively. HR-SEM/TEM, XRD, EDS analysis confirmed the spherical shape, crystalline nature, and elemental formation of silver. Crystallite or grain size was deduced as 5.52 nm for R-AgNPs and 35 nm for S-AgNPs. Zeta-potential indicated electrostatic negative charge for AgNPs, while FTIR revealed the presence of diverse functional groups. Disc diffusion assay indicated the broad-spectrum antibacterial potential of S-AgNPs with the maximum inhibition of B. subtilis while R-AgNPs revealed potency against P. aeruginosa at 10 µg/mL concentration. Biogenic AgNPs revealed antileishmanial activity and the IC 50 was calculated as 164 µg/mL and 184 µg/mL for R-AgNPs and S-AgNPs respectively. Similarly, the R-AgNPs and S-AgNPs revealed anti-cancer potential against HepG2 and the IC 50 was calculated as 49 µg/mL and 69 µg/mL for R-AgNPs and S-AgNPs, respectively. Moreover, the antioxidant activity showed significant results. MTT assay on RD cells, L20B cells, and Hep-2C indicated intensification in viability by reducing the concentration of R-AgNPs and S-AgNPs. The R-AgNPs and S-AgNPs inhibited sabin-like poliovirus (1TCID 50 infection in RD cells). Furthermore, hemocompatibility at low concentrations has been confirmed. Hence, it is concluded that biogenic-AgNPs has the potential to be used in diverse biological applications and that the marine actinobacteria are an excellent resource for fabrication of AgNPs.