Identification of a novel murine glutathione S-transferase class mu gene

Screening of a genomic mouse DNA library with a glutathione S-transferase class mu cDNA probe resulted in the identification of mGSTM5, a novel member of the murine glutathione S-transferase class mu gene family. Here we present the sequence of the promoter region, the exon-intron organization of th...

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Published inBiochemical journal Vol. 330 ( Pt 2); no. 2; pp. 623 - 626
Main Authors De Bruin, W C, Te Morsche, R H, Wagenmans, M J, Alferink, J C, Townsend, A J, Wieringa, B, Peters, W H
Format Journal Article
LanguageEnglish
Published England 01.03.1998
Subjects
Amino Acid Sequence
Animals
Base Sequence
DNA, Complementary - chemistry
Gene Library
Glutathione Transferase - genetics
Liver - enzymology
Mice
Molecular Sequence Data
Multigene Family
Random Amplified Polymorphic DNA Technique
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Summary:Screening of a genomic mouse DNA library with a glutathione S-transferase class mu cDNA probe resulted in the identification of mGSTM5, a novel member of the murine glutathione S-transferase class mu gene family. Here we present the sequence of the promoter region, the exon-intron organization of the gene and the isolation and characterization of its complete cDNA. Conceptual translation of the cDNA sequence revealed that several amino acid positions have been changed in 'invariant' mu class signature sequences in mGSTM5. Reverse transcriptase polymerase chain reaction using gene specific primers revealed that mGSTM5 is uniquely expressed in mouse liver, stomach and small intestine.
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ISSN:0264-6021
1470-8728
DOI:10.1042/bj3300623