Evaluation of Mycobacterium avium Complex Clarithromycin Susceptibility Testing Using SLOMYCO Sensititre Panels and JustOne Strips

• Published in: Journal of Clinical Microbiology Vol. 48; no. 5; pp. 1749 - 1752
• Main Authors: Babady, N. Esther, Hall, Leslie, Abbenyi, Adeline T, Eisberner, Justin J, Brown-Elliott, Barbara A, Pratt, Cody J, McGlasson, Maria C, Beierle, Keanan D, Wohlfiel, Sherri L, Deml, Sharon M, Wallace, Richard J. Jr, Wengenack, Nancy L
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.05.2010; American Society for Microbiology (ASM)
• Subjects: Antitubercular Agents - pharmacology; Clarithromycin - pharmacology; Humans; Microbial Sensitivity Tests - methods; Mycobacteriology and Aerobic Actinomycetes; Mycobacterium avium Complex - drug effects; Mycobacterium avium Complex - isolation & purification; Mycobacterium avium-intracellulare Infection - microbiology
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.01936-09

The SLOMYCO Sensititre panel and the custom JustOne strip (both from TREK Diagnostic Systems, Cleveland, OH) were evaluated for susceptibility testing of Mycobacterium avium complex isolates against clarithromycin. Seventy-one archived and prospectively collected isolates were tested using both the SLOMYCO panel and the JustOne strip, and the results were compared to those obtained using the BACTEC 460 (BD, Sparks, MD) radiometric method and a broth microdilution reference method. Results obtained by the SLOMYCO panel and the JustOne strip agreed with the BACTEC 460 method for 64/71 isolates (90%). Similarly, concordance with the broth microdilution method was 40/43 isolates (93%) for both test systems. The effect of the source medium on inoculum preparation was evaluated, and there were no differences noted in MICs, regardless of whether the inoculum was prepared from isolates grown in Middlebrook 7H9 medium, on Middlebrook 7H10 agar, or in VersaTREK broth culture bottles (Trek Diagnostics). Clarithromycin susceptibility testing of MAC using the SLOMYCO panel and the JustOne strip methods is easy to set up and simple to read and is readily incorporate into the clinical laboratory. These systems offer advantages over the BACTEC 460 system including the lack of a need for radioactive substrates, sharps, or costly instrumentation.