Bacteroides luti sp. nov., an anaerobic, cellulolytic and xylanolytic bacterium isolated from methanogenic sludge

• Published in: International journal of systematic and evolutionary microbiology Vol. 64; no. Pt 5; pp. 1770 - 1774
• Main Authors: Hatamoto, Masashi, Kaneshige, Masami, Nakamura, Akinobu, Yamaguchi, Takashi
• Format: Journal Article
• Language: English
• Published: England 01.05.2014
• Subjects: Bacterial Typing Techniques; Bacteroides - classification; Bacteroides - genetics; Bacteroides - isolation & purification; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Japan; Methane - metabolism; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sewage - microbiology
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.056630-0

A mesophilic, anaerobic, cellulolytic and xylanolytic strain, UasXn-3T, was isolated from anaerobic granular sludge in a mesophilic upflow anaerobic sludge blanket reactor, which was used to treat municipal sewage. The cells were Gram-stain-negative, non-motile, and non-spore-forming rods. The optimal temperature for growth was 37-40 °C and the optimal pH for growth was pH 6.5-7.0. Strain UasXn-3T could grow on several polysaccharides and sugars, including cellulose, cellobiose, xylan, xylose, glucose, fructose, arabinose, mannose, raffinose, trehalose and starch. The DNA G+C content was 44.4 mol%. On the basis of comparative 16S rRNA gene sequence analysis, strain UasXn-3T was identified as a member of the genus Bacteroides and most closely related to Bacteroides oleiciplenus, B. intestinalis, B. cellulosilyticus and B. graminisolvens (sequence similarities of 91.3-91.6%). Since the genetic and phenotypic properties suggest that strain UasXn-3T represents a novel species, we propose the name Bacteroides luti sp. nov. The type strain is UasXn-3T (=JCM 19020T=DSM 26991T).