A novel prostate carcinoma-associated glycoprotein complex (PAC) recognized by monoclonal antibody TURP-27

• Published in: International journal of cancer Vol. 47; no. 5; p. 717
• Main Authors: Wright, Jr, G L, Beckett, M L, Lipford, G B, Haley, C L, Schellhammer, P F
• Format: Journal Article
• Language: English
• Published: United States 12.03.1991
• Subjects: Adenocarcinoma - immunology; Antibodies, Monoclonal; Antigens, Neoplasm - analysis; Antigens, Neoplasm - chemistry; Biomarkers, Tumor - analysis; Blotting, Western; Humans; Immunoenzyme Techniques; Male; Prostate - immunology; Prostate-Specific Antigen; Prostatic Neoplasms - immunology; Radioimmunoassay
• ISSN: 0020-7136; 1097-0215
• DOI: 10.1002/ijc.2910470516

A prostate carcinoma-associated antigen recognized by MAb TURP-27 was characterized immunohistochemically and biochemically. TURP-27 antigen was found localized in the cell membrane and cytoplasm of the ductal epithelial cells of normal (10%), benign (75-100%) and malignant (20-100%) prostate cells. Fetal prostate tissues were also found to express the TURP-27 antigen, suggesting expression early in development. This antigen was not expressed by non-prostate tumors examined, but significant cross-reactivity was observed in myelinated nerves while minor cross-reactivity was seen in certain lymphocyte subsets, cells in the adrenal medulla and chief cells of stomach. Immunoblotting and biochemical data demonstrated that the TURP-27 antigen is a sialic-acid-containing glycoprotein complex with major molecular species in prostate tissues of 310-250, 180, 140, 115, 95-90, 69, and 40- to 35-kDa. Immunoblotting patterns similar to those observed for prostate tissues were also seen in CNS extracts with the exception of the 69 and 40- to 35-kDa proteins. This prostate carcinoma-associated sialoglycoprotein complex (PAC) recognized by MAb TURP-27 is likely to represent a novel tumor antigen expressed by prostate tumors.