No Association Between the Polymorphisms in Matrix Metalloproteinase-1 and Matrix Metalloproteinase-3 Promoter Regions and Colorectal Cancer in Chinese

• Published in: Diseases of the colon & rectum Vol. 49; no. 9; pp. 1439 - 1444
• Main Authors: Xu, Enping, Lai, Maode, Lŭ, Bingjian, Xing, Xiaoming, Huang, Qiong
• Format: Journal Article
• Language: English
• Published: Secaucus, NJ The ASCRS 01.09.2006; Springer; Lippincott Williams & Wilkins Ovid Technologies
• Subjects: Biological and medical sciences; Case-Control Studies; China; Colorectal Neoplasms - genetics; Female; Gastroenterology. Liver. Pancreas. Abdomen; Genetic Predisposition to Disease; Genotype; Haplotypes; Humans; Male; Matrix Metalloproteinase 1 - genetics; Matrix Metalloproteinase 3 - genetics; Medical sciences; Middle Aged; Polymorphism, Genetic; Promoter Regions, Genetic - genetics; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; China; Rectal disease; Enzyme; Colorectal cancer; Matrix metalloproteinase-1; Metalloendopeptidases; Malignant tumor; Colonic disease; Promoter; Peptidases; Matrix metalloproteinase-3; Gastroenterology; Nucleotide; Digestive diseases; Hydrolases; Intestinal disease; Single nucleotide polymorphism; Interstitial collagenase; Polymorphism
• ISSN: 0012-3706; 1530-0358
• DOI: 10.1007/s10350-006-0652-9

Matrix metalloproteinase-1 and matrix metalloproteinase-3 are implicated in all steps of cancer initiation, invasion, and metastasis. Recently, several genetic studies have demonstrated that matrix metalloproteinase-1-1607 ins/delG (1G/2G) polymorphism and matrix metalloproteinase-3-1612 ins/delA (5A/6A) polymorphism modify each transcriptional activity in allele-specific manners. In this study, we investigated whether these functional polymorphisms are associated with colorectal cancer in a Chinese population. Matrix metalloproteinase-1 and matrix metalloproteinase-3 genotypings were performed on 126 pathologically diagnosed colorectal cancer patients and 126 age-matched and gender-matched controls by polymerase chain reaction-based denaturing high-performance liquid chromatography analysis or restriction fragment length polymorphism, respectively. The distributions of the matrix metalloproteinase-1 and matrix metalloproteinase-3 genotypes in healthy control subjects were inconsistent with Hardy-Weinberg equilibrium. Neither the genotype frequencies nor allele frequencies of matrix metalloproteinase-1 and matrix metalloproteinase-3 polymorphisms showed significant difference from those in healthy control subjects. There also were no significant associations between matrix metalloproteinase-1 and matrix metalloproteinase-3 genotypes and clinicopathologic features. When we examined the linkage disequilibrium between these two single nucleotide polymorphisms using expectation-maximization algorithm, we found that the two single nucleotide polymorphisms were in a strong linkage disequilibrium, but no significant difference was found in haplotype distribution. Our present data suggest that the matrix metalloproteinase-1 and matrix metalloproteinase-3 promoter polymorphisms may not be useful markers to predicate susceptibility of colorectal cancer in Chinese.